Schmitz H, von Deimling U, Flehmig B
J Gen Virol. 1980 Sep;50(1):59-68. doi: 10.1099/0022-1317-50-1-59.
We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.
我们应用了过氧化物酶标记抗原（ELA）来检测巨细胞病毒IgM抗体：微量滴定板用抗IgM免疫球蛋白包被。人血清中的IgM部分选择性地结合到预先包被的板上，然后通过酶标记抗原检测病毒特异性IgM抗体。本文描述了一种标记病毒抗原的高效技术。IgM抗体的检测简单且特异，类风湿因子IgM不干扰该试验。
