Tedder R S, Yao J L, Anderson M J
J Hyg (Lond). 1982 Apr;88(2):335-50. doi: 10.1017/s0022172400070182.
Mice were immunized by three intraperitoneal and one intravenous injection of rubella haemagglutinin. Splenocytes from these mice were fused with the cells of a syngeneic myeloma cell line, and following culture for various periods of time, single-cell clones were derived by the technique of limiting dilution. A total of 139 clones were derived from 13 parent hybrid cultures. To date, four of these cloned cultures have been propagated as ascitic tumours in mice. The preparation of IgG from ascitic fluid and labelling of this antibody with 125I is described. Results indicate that the use of labelled monoclonal antibodies as indicator reagents in solid-phase IgM antibody capture assays for the detection of rubella-specific IgM results in enhanced performance of these tests.
用风疹血凝素对小鼠进行三次腹腔注射和一次静脉注射免疫。将这些小鼠的脾细胞与同基因骨髓瘤细胞系的细胞融合，在培养不同时间后，通过有限稀释技术获得单细胞克隆。从13个亲本杂交培养物中总共获得了139个克隆。迄今为止，其中4个克隆培养物已在小鼠体内作为腹水瘤进行传代培养。描述了从腹水中制备IgG并将该抗体用125I标记的方法。结果表明，在用于检测风疹特异性IgM的固相IgM抗体捕获试验中，使用标记的单克隆抗体作为指示试剂可提高这些试验的性能。
