系统性红斑狼疮中的C型肿瘤病毒分离研究。I. 通过等密度沉降法对3H-尿苷标记病毒粒子进行检测的尝试
Type C oncornavirus isolation studies in systemic lupus erythematosus. I. Attempted detection by isopycnic sedimentation of 3H-uridine-labelled virions.
作者信息
Phillips P E, Hargrave R, Stewart E, Sarkar N H
出版信息
Ann Rheum Dis. 1975 Oct;35(5):422-8. doi: 10.1136/ard.35.5.422.
Abstract
Type C oncornavirus isolation was attempted from cell cultures of tissues from 7 patients with systemic lupus erythematosus. Detection was based on the characteristic sedimentation of 3H-uridine-labelled virions at a density of 1-16 g/ml. Cultures positive by this method were negative by two other criteria for type C viruses: characteristic virions by electron microscopy and the viral enzyme RNA-directed DNA polymerase. The positive results were probably due to cellular damage by prolonged radiolabelling, with release of organelles containing labelled RNA sedimenting at the same density as type C viruses.
摘要
尝试从7例系统性红斑狼疮患者组织的细胞培养物中分离C型肿瘤病毒。检测基于3H-尿苷标记的病毒体在密度为1-16 g/ml时的特征性沉降。通过该方法呈阳性的培养物，根据C型病毒的另外两条标准判断为阴性：电子显微镜下的特征性病毒体以及病毒酶RNA指导的DNA聚合酶。阳性结果可能是由于长时间放射性标记导致细胞损伤，释放出含有标记RNA的细胞器，其沉降密度与C型病毒相同。
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