大鼠脂肪细胞中对N-乙基马来酰亚胺敏感和不敏感形式的甘油磷酸酰基转移酶的亚细胞分布及某些特性
Subcellular distribution and some properties of N-ethylmaleimide-sensitive and-insensitive forms of glycerol phosphate acyltransferase in rat adipocytes.
作者信息
Saggerson E D, Carpenter C A, Cheng C H, Sooranna S R
出版信息
Biochem J. 1980 Jul 15;190(1):183-9. doi: 10.1042/bj1900183.
Abstract
- Glycerol phosphate acyltransferase (GPAT) activities were measured in subcellular fractions obtained from rat epididymal adipocytes. These contained both N-ethylmaleimide-sensitive and N-ethylmaleimide-insensitive forms of the enzyme. 2. As shown by parallel measurements of marker enzymes, N-ethylmaleimide-insensitive GPAT is most probably a mitochondrial activity, whereas N-ethylmaleimide-sensitive GPAT is the microsomal enzyme. 3. Subcellular distributions are also reported for dihydroxyacetone phosphate acyltransferase (DHAPAT) (assayed with and without N-ethylmaleimide), monoacylglycerol phosphate acyltransferase (MGPAT) and Mg2+-dependent and Mg2+-independent forms of phosphatidate phosphohydrolase (PPH).
摘要
- 测定了从大鼠附睾脂肪细胞获得的亚细胞组分中的甘油磷酸酰基转移酶（GPAT）活性。这些组分中含有该酶的N-乙基马来酰亚胺敏感型和N-乙基马来酰亚胺不敏感型。2. 如通过对标记酶的平行测量所示，N-乙基马来酰亚胺不敏感的GPAT很可能是一种线粒体活性，而N-乙基马来酰亚胺敏感的GPAT是微粒体酶。3. 还报道了磷酸二羟丙酮酰基转移酶（DHAPAT）（在有和没有N-乙基马来酰亚胺的情况下进行测定）、单酰甘油磷酸酰基转移酶（MGPAT）以及Mg2+依赖性和Mg2+非依赖性形式的磷脂酸磷酸水解酶（PPH）的亚细胞分布。
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