大鼠脂肪组织中脂蛋白脂肪酶及参与三酰甘油合成的酶的活性。饥饿、饮食改变及促肾上腺皮质激素注射的影响。

The activities of lipoprotein lipase and of enzymes involved in triacylglycerol synthesis in rat adipose tissue. Effects of starvation, dietary modification and of corticotropin injection.

作者信息

Lawson N, Pollard A D, Jennings R J, Gurr M I, Brindley D N

出版信息

Biochem J. 1981 Nov 15;200(2):285-94. doi: 10.1042/bj2000285.

DOI:10.1042/bj2000285

PMID:6280682

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163534/

Abstract

The effects of dietary modification, including starvation, and of corticotropin injection on the activities of acyl-CoA synthetase, glycerol phosphate acyltransferase, dihydroxyacetone phosphate acyltransferase, phosphatidate phosphohydrolase, diacylglycerol acyltransferase and lipoprotein lipase were measured in adipose tissue. 2. Lipoprotein lipase activities in heart were increased and those in adipose tissue were decreased when rats were fed on diets enriched with corn oil or beef tallow rather than with sucrose or starch. The lipoprotein lipase activity was lower in the adipose tissue of rats fed on the sucrose rather than on the starch diet. 3. Rats fed on the beef tallow diet had slightly higher activities of the total glycerol phosphate acyltransferase in adipose tissue than did rats fed on the sucrose or starch diet. The diacylglycerol acyltransferase and the mitochondrial glycerol phosphate acyltransferase activities were higher for the rats fed on the tallow diet than for those fed on the corn-oil diet. 4. Starvation significantly decreased the activities of lipoprotein lipase (after 24 and 48 h), acyl-CoA synthetase (after 24 h) and of the mitochondrial glycerol phosphate acyltransferase and the N-ethylmaleimide-insensitive dihydroxyacetone phosphate acyltransferase (after 48 h) in adipose tissue. The activities of the microsomal glycerol phosphate acyltransferase, diacylglycerol acyltransferase and the soluble phosphatidate phosphohydrolase were not significantly changed after 24 or 48 h of starvation. 5. The activities of lipoprotein lipase and phosphatidate phosphohydrolase in adipose tissue were decreased 15 min after corticotropin was injected into rats during November to December. No statistically significant differences were found when these experiments were performed during March to September. These differences may be related to the seasonal variation in acute lipolytic responses. 6. These results are discussed in relation to the control of triacylglycerol synthesis and lipoprotein metabolism.

摘要

在脂肪组织中测定了饮食调整（包括饥饿）以及注射促肾上腺皮质激素对酰基辅酶A合成酶、甘油磷酸酰基转移酶、磷酸二羟丙酮酰基转移酶、磷脂酸磷酸水解酶、二酰基甘油酰基转移酶和脂蛋白脂肪酶活性的影响。2. 当给大鼠喂食富含玉米油或牛脂的饲料而非蔗糖或淀粉饲料时，心脏中的脂蛋白脂肪酶活性增加，而脂肪组织中的脂蛋白脂肪酶活性降低。喂食蔗糖而非淀粉饲料的大鼠脂肪组织中的脂蛋白脂肪酶活性较低。3. 喂食牛脂饲料的大鼠脂肪组织中总甘油磷酸酰基转移酶的活性略高于喂食蔗糖或淀粉饲料的大鼠。喂食牛脂饲料的大鼠的二酰基甘油酰基转移酶和线粒体甘油磷酸酰基转移酶活性高于喂食玉米油饲料的大鼠。4. 饥饿显著降低了脂肪组织中脂蛋白脂肪酶（24小时和48小时后）、酰基辅酶A合成酶（24小时后）以及线粒体甘油磷酸酰基转移酶和N - 乙基马来酰亚胺不敏感的磷酸二羟丙酮酰基转移酶（48小时后）的活性。饥饿24小时或48小时后，微粒体甘油磷酸酰基转移酶、二酰基甘油酰基转移酶和可溶性磷脂酸磷酸水解酶的活性没有显著变化。5. 在11月至12月期间给大鼠注射促肾上腺皮质激素15分钟后，脂肪组织中脂蛋白脂肪酶和磷脂酸磷酸水解酶的活性降低。在3月至9月期间进行这些实验时，未发现统计学上的显著差异。这些差异可能与急性脂解反应的季节性变化有关。6. 结合三酰甘油合成和脂蛋白代谢调控对这些结果进行了讨论。