Il'ina T S, Nechaeva E V, Smirnov G B
Genetika. 1980;16(6):957-66.
The method allowing the induction of bacterial mutations affecting Tn9 transposition from the bacteriophage genome to the Escherichia coli chromosome is described. Neither impaired ability of cells to adsorb bacteriophages, nor phage DNA degradation in the mutant cells were observed in the transposition-defective mutants isolated by the method. This led us to the conclusion that the isolated mutants were indeed defective in the transposition of Tn9.
本文描述了一种诱导细菌突变的方法,该突变会影响噬菌体基因组到大肠杆菌染色体的Tn9转座。通过该方法分离出的转座缺陷型突变体中,未观察到细胞吸附噬菌体的能力受损,也未观察到突变体细胞中的噬菌体DNA降解。这使我们得出结论,所分离出的突变体在Tn9转座方面确实存在缺陷。