Ilyina T S, Nechaeva E V, Smirnov G B
Mol Gen Genet. 1981;181(3):384-9. doi: 10.1007/BF00425616.
Five mutants (called tnm) of Escherichia coli with impaired ability for transposition of Tn9 were isolated after treatment with ethyl methanesulfonate (EMS) or N-methyl-N'-nitro-N-nitrosoguanidine (NG). The map locations of the tnm mutations were determined by a combination of Hfr matings, F' episome complementation and P1 transductional mapping. The data obtained show that the five tnm mutations are located near 91 min on the Escherichia coli linkage map and are cotransducible with the metA marker with a frequency of 3%-4%. Introduction of F' plasmids containing this region complements the Tnm- phenotype for the two mutants tested i.e. tnm-1 and tnm-2 are recessive in tnm+/tnm- merodiploids.
在用甲磺酸乙酯（EMS）或N-甲基-N'-硝基-N-亚硝基胍（NG）处理后，分离出了5株转座能力受损的大肠杆菌突变体（称为tnm）。通过高频重组（Hfr）杂交、F'附加体互补和P1转导作图相结合的方法确定了tnm突变的图谱位置。所得数据表明，这5个tnm突变位于大肠杆菌连锁图谱上91分钟附近，与metA标记共转导的频率为3%-4%。导入含有该区域的F'质粒可互补所测试的两个突变体的Tnm-表型，即在tnm+/tnm-部分二倍体中，tnm-1和tnm-2是隐性的。
