Ho M K, Morse S I, Kong A S
J Exp Med. 1981 Jan 1;153(1):75-88. doi: 10.1084/jem.153.1.75.
Pertussis B mitogen (PBM), isolated from culture supernatant fluids of Bordetella pertussis, is a potent mitogen for mouse and human lymphocytes. In mice, > 95% of the blast cells recovered from PBM cultures bear surface immunoglobulins. Therefore, PBM seems to induce proliferation of mouse B lymphocytes, but not T cells. The proliferative response observed is nonspecific because cells from all mouse strains tested, including germfree animals, are responsive. Moreover, the mitogenic activity of PBM is independent of T lymphocytes, macrophages, or serum factors. When human peripheral blood or cord blood lymphocytes are cultured in the presence of PBM, a high level of thymidine incorporation by these cells is detected. Furthermore, PBM can induce polyclonal antibody formation by both mouse and human lymphocytes. Despite similar methods of isolation, PBM is distinct from the lymphocytosis-promoting factor of B. pertussis, a previously described T cell mitogen.
百日咳杆菌B促有丝分裂原(PBM)是从百日咳博德特氏菌培养上清液中分离出来的,是一种对小鼠和人类淋巴细胞有效的促有丝分裂原。在小鼠中,从PBM培养物中回收的超过95%的母细胞带有表面免疫球蛋白。因此,PBM似乎能诱导小鼠B淋巴细胞增殖,但不能诱导T细胞增殖。观察到的增殖反应是非特异性的,因为所有测试的小鼠品系的细胞,包括无菌动物的细胞,都有反应。此外,PBM的促有丝分裂活性不依赖于T淋巴细胞、巨噬细胞或血清因子。当在PBM存在的情况下培养人外周血或脐带血淋巴细胞时,可检测到这些细胞高水平的胸苷掺入。此外,PBM可诱导小鼠和人类淋巴细胞形成多克隆抗体。尽管分离方法相似,但PBM与百日咳博德特氏菌的淋巴细胞增多促进因子不同,后者是先前描述的一种T细胞促有丝分裂原。