Drummond R J, Dean G
J Neurochem. 1980 Nov;35(5):1155-65. doi: 10.1111/j.1471-4159.1980.tb07871.x.
Wolfgram protein preparations from myelin are heterogeneous mixtures having major components with molecular weights in the 43,000 to 60,000 range. Through the use of an SDS-slab gel electrophoretic system of high resolving power, the Wolfgram 1 (W1) component described by Nussbaum et al. (1977) has been resolved into two components; bovine W1a and W1b proteins are found to have mobilities identical to those of the two 2',3'-cyclic nucleotide 3'-phosphodiesterase (2'3'-CN 3'-ase) components, CNa and CNb (Drummond, 1979). Crossed immunoelectrophoresis with bovine 2',3'-CN 3'-ase demonstrates that CNa and CNb are antigenically indistinguishable. Rat and bovine Wolfgram proteins W1a and W1b, but not the W2 proteins, display cross reactivity with antiserum directed against bovine 2',3'-CN 3'-ase. Chymotryptic and Staphylococcus aureus V8 protease peptide profiles indicate structural similarities between proteins CNa-W1a and CNb-W1b. It is concluded that an inactivated form of 2',3'-Cn 3'-ase is a major component of Wolfgram protein W1. These results are consistent with the studies on the histochemical localization of rat W1 protein by Roussel et al. (1977, 1978).
来自髓磷脂的沃尔夫格拉姆蛋白质制剂是异质混合物，其主要成分的分子量在43,000至60,000范围内。通过使用具有高分辨力的SDS平板凝胶电泳系统，努斯鲍姆等人（1977年）描述的沃尔夫格拉姆1（W1）成分已被分解为两个成分；发现牛W1a和W1b蛋白的迁移率与两种2',3'-环核苷酸3'-磷酸二酯酶（2'3'-CN 3'-ase）成分CNa和CNb的迁移率相同（德拉蒙德，1979年）。用牛2',3'-CN 3'-ase进行交叉免疫电泳表明，CNa和CNb在抗原性上无法区分。大鼠和牛的沃尔夫格拉姆蛋白W1a和W1b，而不是W2蛋白，与针对牛2',3'-CN 3'-ase的抗血清显示出交叉反应性。胰凝乳蛋白酶和金黄色葡萄球菌V8蛋白酶肽谱表明蛋白质CNa-W1a和CNb-W1b之间存在结构相似性。得出的结论是，2',3'-Cn 3'-ase的一种失活形式是沃尔夫格拉姆蛋白W1的主要成分。这些结果与鲁塞尔等人（1977年、1978年）对大鼠W1蛋白的组织化学定位研究一致。
