Thorén M, Ajne M, Hall K
Acta Endocrinol (Copenh). 1981 Jan;96(1):15-23. doi: 10.1530/acta.0.0960015.
A radioimmunoassay for ACTH determinations in human plasma was developed using an N-terminal antiserum. Plasma ACTH levels at 6 a.m. in 39 patients with normal pituitary-adrenal function were below 4 pmol/l in two individuals. The remaining had a mean of 32.7 +/- 4 pmol/l (n = 37). Gel filtration on Sephadex G-50 medium and G-75 revealed that a high molecular weight immunoreactive ACTH was found in plasma from both normal individuals and patients with Nelson's syndrome. The stimulation of ACTH secretion with metyrapone (750 mg perorally) significantly increased the mean plasma levels of 1-39 ACTH after 3 h (from 0.9 to 6.6 pmol/l) in 10 healthy individuals. The level of the high molecular weight form remained constant. In one patient with Nelson's syndrome hydrocortisone iv (100 mg/h for 3 h) suppressed the total ACTH levels from 360 to 83 pmol/l following corticoid withdrawal on the previous evening, but did not affect the high molecular weight form of ACTH. The difference between the amounts of immunoreactive ACTH found in plasma from normal individuals and from patients with Nelson's syndrome was attributed to 1-39 ACTH.
利用 N 端抗血清开发了一种用于测定人血浆中促肾上腺皮质激素（ACTH）的放射免疫分析法。39 例垂体 - 肾上腺功能正常的患者在上午 6 点时，有 2 例血浆 ACTH 水平低于 4 pmol/L。其余患者（n = 37）的平均水平为 32.7±4 pmol/L。在 Sephadex G - 50 介质和 G - 75 上进行凝胶过滤显示，正常个体和尼尔森综合征患者的血浆中均发现了一种高分子量的免疫反应性 ACTH。在 10 名健康个体中，用甲吡酮（口服 750 mg）刺激 ACTH 分泌 3 小时后，1 - 39 ACTH 的平均血浆水平显著升高（从 0.9 升至 6.6 pmol/L）。高分子量形式的水平保持不变。在 1 例尼尔森综合征患者中，在前一晚停用皮质类固醇后，静脉注射氢化可的松（100 mg/h，共 3 小时）使总 ACTH 水平从 360 降至 83 pmol/L，但不影响 ACTH 的高分子量形式。正常个体和尼尔森综合征患者血浆中发现的免疫反应性 ACTH 量的差异归因于 1 - 39 ACTH。
