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长期垂体切除大鼠结节部前叶垂体激素的免疫细胞化学证明

Immunocytochemical demonstration of anterior pituitary hormones in the pars tuberalis of long-term hypophysectomized rats.

作者信息

Ordronneau P, Petrusz P

出版信息

Am J Anat. 1980 Aug;158(4):491-506. doi: 10.1002/aja.1001580410.

DOI:10.1002/aja.1001580410
PMID:6257103
Abstract

The hormone-containing cells of the pars tuberalis of the rat adenohypophysis were studied, 4--5 months after hypophysectomy, with a double bridge immunocytochemical technique and with antisera directed against the hormones of the pars distalis. Corticotropes, lactotropes, somatotropes, thyrotropes, and gonadotropes were found in the pars tuberalis of all animals. These cells are well well situated to receive blood, nerve terminals, and tanycyte processes from the median eminence and, thus, to be influenced by the chemical messengers from the hypothalamus. It is concluded that the pars tuberalis has to be regarded as a potential source of adenohypophyseal hormones in an animal model traditionally considered to be free of such hormones.

摘要

采用双桥免疫细胞化学技术和针对腺垂体远侧部激素的抗血清,对大鼠腺垂体结节部含激素细胞进行了研究,研究是在垂体切除术后4 - 5个月进行的。在所有动物的结节部均发现了促肾上腺皮质激素细胞、催乳激素细胞、生长激素细胞、促甲状腺激素细胞和促性腺激素细胞。这些细胞所处位置良好,能够接受来自正中隆起的血液、神经末梢和伸长细胞突起,因此会受到来自下丘脑化学信使的影响。得出的结论是,在传统上认为不含此类激素的动物模型中,结节部应被视为腺垂体激素的潜在来源。

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Immunocytochemical demonstration of anterior pituitary hormones in the pars tuberalis of long-term hypophysectomized rats.长期垂体切除大鼠结节部前叶垂体激素的免疫细胞化学证明
Am J Anat. 1980 Aug;158(4):491-506. doi: 10.1002/aja.1001580410.
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The hypophyseal pars tuberalis is enriched with distinct phosphotyrosine-containing proteins not detected in other areas of the brain and pituitary.垂体结节部富含在脑和垂体其他区域未检测到的独特含磷酸酪氨酸蛋白。
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Criteria of reliability for light microscopic immunocytochemical staining.
光学显微镜免疫细胞化学染色的可靠性标准。
Histochem J. 1980 May;12(3):333-48. doi: 10.1007/BF01006954.
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Corticotropin-releasing factor (CRF)-like immunoreactivity in the vertebrate endocrine pancreas.脊椎动物内分泌胰腺中促肾上腺皮质激素释放因子(CRF)样免疫反应性
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The pars tuberalis of the human pituitary. A histologic, immunohistochemical, ultrastructural and immunoelectron microscopic analysis.人类垂体结节部。组织学、免疫组织化学、超微结构及免疫电镜分析。
Virchows Arch A Pathol Anat Histopathol. 1983;399(1):49-59. doi: 10.1007/BF00666218.
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Histochem J. 1983 Mar;15(3):189-200. doi: 10.1007/BF01006235.
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