无症状吸烟者肺泡巨噬细胞体外氧化代谢反应的改变。

Altered oxidative metabolic responses in vitro of alveolar macrophages from asymptomatic cigarette smokers.

作者信息

Hoidal J R, Fox R B, LeMarbe P A, Perri R, Repine J E

出版信息

Am Rev Respir Dis. 1981 Jan;123(1):85-9. doi: 10.1164/arrd.1981.123.1.85.

DOI:10.1164/arrd.1981.123.1.85

Abstract

Superoxide anion (O2) release by alveolar macrophages (AM) from young asymptomatic cigarette smokers was greater than that by AM from age-matched nonsmokers. Greater O2 release by AM from cigarette smokers was observed before and after stimulation by bacteria or phorbol myristate acetate (PMA). In contrast, oxygen uptake and glucose (1(-14)C) oxidation by unstimulated or stimulated AM from cigarette smokers was the same as that by AM from non-smokers. The selective increase of O2 release by AM from cigarette smokers was not due to a lack of O2 scavenging agent within the cells, since intracellular superoxide dismutase (SOD) was increased in AM from smokers. The potential importance of enhanced O2 release by AM from cigarette smokers was confirmed by demonstrating that lysis of fibroblasts induced by AM from smokers was completely prevented by addition of SOD and catalase.

摘要

年轻无症状吸烟者肺泡巨噬细胞（AM）释放的超氧阴离子（O2）比年龄匹配的非吸烟者的AM释放的超氧阴离子更多。在细菌或佛波酯肉豆蔻酸酯（PMA）刺激之前和之后，均观察到吸烟者的AM释放更多的O2。相比之下，吸烟者未受刺激或受刺激的AM的氧摄取和葡萄糖（1(-14)C）氧化与非吸烟者的AM相同。吸烟者的AM释放O2的选择性增加并非由于细胞内缺乏O2清除剂，因为吸烟者的AM中细胞内超氧化物歧化酶（SOD）增加。通过证明添加SOD和过氧化氢酶可完全防止吸烟者的AM诱导的成纤维细胞溶解，证实了吸烟者的AM增强释放O2的潜在重要性。