Olson J E, Schimmerling W, Tobias C A
Brain Res. 1981 Jan 12;204(2):436-40. doi: 10.1016/0006-8993(81)90604-1.
The change in excitability of unstained nerve cells from neonatal rat cerebellum was measured as a function of the energy flux and wavelength of incident laser light. The energy flux was in the range of 0 to 100 microJ/sq. microns. 6 wavelengths between 490 and 685 nm were used. Laser pulses above a threshold energy flux significantly reduced the cells' excitability as measured by extracellular stimulation. The sensitivity of the cells, defined as the inverse of the threshold energy density, increased by an order of magnitude toward the shorter wavelengths. These results are consistent with primary absorption of the light by mitochondrial enzymes, resulting in local heating followed by mitochondrial calcium release into the cytoplasm.
将新生大鼠小脑未染色神经细胞的兴奋性变化作为入射激光能量通量和波长的函数进行测量。能量通量范围为0至100微焦/平方微米。使用了490至685纳米之间的6种波长。高于阈值能量通量的激光脉冲显著降低了通过细胞外刺激测量的细胞兴奋性。细胞的敏感性定义为阈值能量密度的倒数，朝着较短波长增加了一个数量级。这些结果与线粒体酶对光的初级吸收一致，导致局部加热，随后线粒体钙释放到细胞质中。
