在大肠杆菌K-12中对ksgB和一个新的Tn5诱导的卡那霉素抗性基因ksgD进行转导作图。
Transductional mapping of ksgB and a new Tn5-induced kasugamycin resistance gene, ksgD, in Escherichia coli K-12.
作者信息
Fouts K E, Barbour S D
出版信息
J Bacteriol. 1981 Feb;145(2):914-9. doi: 10.1128/jb.145.2.914-919.1981.
Abstract
We have mapped the Escherichia coli ksgB gene to min 36.5, 0.8 min from man and 0.7 min from aroD. A new kasugamycin resistance (Ksgr) gene, ksgD, has been isolated, using a transposon, Tn5. ksgD::TN5 is 44% cotransducible with sbcA, unlinked to trp, and unlinked to man (by P1 transduction). The ksgD::Tn5 has a late time of entry from HfrB7 (PO43). These data place ksgD clockwise from sbcA (which enters early from HfrB7) at min 30.4. The reistance of ksgB ksgD single and double mutant strains has been quantitated. Single mutations, ksgB or ksgD, gave resistance to 600 micrograms of kasugamycin per ml, whereas a ksgB ksgD strain was able to grow in the presence of kasugamycin levels in excess of 3,000 micrograms/ml. This indicates that the mechanisms of resistance coded for by the two genes are independent and synergistic.
摘要
我们已将大肠杆菌ksgB基因定位到36.5分钟处，距man基因0.8分钟，距aroD基因0.7分钟。利用转座子Tn5分离出了一个新的卡那霉素抗性（Ksgr）基因ksgD。ksgD::TN5与sbcA的共转导率为44%，与trp不连锁，与man不连锁（通过P1转导）。ksgD::Tn5从HfrB7（PO43）进入的时间较晚。这些数据表明ksgD位于sbcA（从HfrB7早期进入）顺时针方向30.4分钟处。已对ksgB ksgD单突变和双突变菌株的抗性进行了定量。单突变，ksgB或ksgD，对每毫升600微克卡那霉素具有抗性，而ksgB ksgD菌株能够在卡那霉素浓度超过3000微克/毫升的情况下生长。这表明这两个基因编码的抗性机制是独立且协同的。
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