Rubin R A, Modrich P, Vanaman T C
J Biol Chem. 1981 Mar 10;256(5):2140-2.
NH2- and COOH-terminal amino acid sequences of the Eco RI restriction and modification enzymes have been determined. The results allow localization of the coding regions within the DNA segment which controls activity of both enzymes. Processing of the endonuclease is limited to removal of NH2-terminal formylmethionine whereas, in the case of the methylase, formylMet-Ala is removed.
已确定了Eco RI限制酶和修饰酶的氨基末端和羧基末端氨基酸序列。这些结果有助于确定控制这两种酶活性的DNA片段内的编码区域。核酸内切酶的加工仅限于去除氨基末端的甲酰甲硫氨酸，而对于甲基化酶，则去除甲酰甲硫氨酸-丙氨酸。
