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枯草芽孢杆菌噬菌体SPR的序列特异性DNA甲基转移酶编码基因的结构

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.

作者信息

Pósfai G, Baldauf F, Erdei S, Pósfai J, Venetianer P, Kiss A

出版信息

Nucleic Acids Res. 1984 Dec 11;12(23):9039-49. doi: 10.1093/nar/12.23.9039.

DOI:10.1093/nar/12.23.9039
PMID:6096817
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320436/
Abstract

The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.

摘要

采用马克萨姆-吉尔伯特法测定了枯草芽孢杆菌噬菌体SPR编码5'-GGCC和5'-CCGG特异性DNA甲基转移酶的基因的核苷酸序列。借助S1作图和在大肠杆菌小细胞中的翻译对该序列的转录和翻译信号进行了定位。该基因编码一种49kd的多肽。SPR甲基化酶的氨基酸序列显示出与5'-GGCC特异性BspRI修饰甲基化酶的序列具有同源区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a46a/320436/7924f1c8d7ce/nar00341-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a46a/320436/7924f1c8d7ce/nar00341-0300-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a46a/320436/7924f1c8d7ce/nar00341-0300-a.jpg

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