大肠杆菌DNA腺嘌呤甲基化酶（dam）基因的分离与鉴定。
The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.
作者信息
Brooks J E, Blumenthal R M, Gingeras T R
出版信息
Nucleic Acids Res. 1983 Feb 11;11(3):837-51. doi: 10.1093/nar/11.3.837.
Abstract
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms.
摘要
已知大肠杆菌的dam（DNA腺嘌呤甲基化酶）可使GATC序列发生甲基化。运用一种克隆序列特异性DNA甲基化酶基因的通用方法，在插入质粒载体pBR322的1.14 kb片段上分离出了dam基因。随后的限制性酶切图谱分析和亚克隆实验确定了该基因的一组大致边界。测定了dam基因的核苷酸序列，对该序列的分析揭示了一个独特的开放阅读框，其长度与编码dam大小蛋白质所需的长度一致。从该序列推导的氨基酸组成与纯化的dam蛋白的氨基酸组成密切对应。采用酶学和DNA:DNA杂交方法研究了多种原核生物中可能存在的dam基因。
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本文引用的文献
1
SEQ: a nucleotide sequence analysis and recombination system.SEQ：一种核苷酸序列分析与重组系统。
Nucleic Acids Res. 1982 Jan 11;10(1):279-94. doi: 10.1093/nar/10.1.279.
2
A semi-automated method for the reading of nucleic acid sequencing gels.一种用于读取核酸测序凝胶的半自动方法。
Nucleic Acids Res. 1982 Jan 11;10(1):103-14. doi: 10.1093/nar/10.1.103.
3
The nucleotide sequence of the structural gene for Escherichia coli tryptophanyl-tRNA synthetase.大肠杆菌色氨酰 - tRNA合成酶结构基因的核苷酸序列。
J Biol Chem. 1982 Jun 10;257(11):6132-6.
4
Primary structure of the chromosomal origins (oriC) of Enterobacter aerogenes and Klebsiella pneumoniae: comparisons and evolutionary relationships.产气肠杆菌和肺炎克雷伯菌染色体起源（oriC）的一级结构：比较与进化关系。
J Bacteriol. 1982 Jun;150(3):1467-71. doi: 10.1128/jb.150.3.1467-1471.1982.
5
Escherichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme.大肠杆菌DNA腺嘌呤甲基化酶。纯酶的物理性质和催化特性。
J Biol Chem. 1982 Mar 10;257(5):2605-12.
6
Escherichia coli K-12 clones that overproduce dam methylase are hypermutable.过量产生dam甲基化酶的大肠杆菌K-12克隆具有高度可突变性。
J Bacteriol. 1981 Jan;145(1):644-6. doi: 10.1128/jb.145.1.644-646.1981.
7
Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.缺乏甲基化指导的DNA错配修复的大肠杆菌突变体
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7. doi: 10.1073/pnas.77.2.1063.
8
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9
A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis.一种用于分离适合限制性内切酶分析的重组质粒DNA的快速微量技术。
Plasmid. 1980 Jan;3(1):88-91. doi: 10.1016/s0147-619x(80)90037-2.
10
Modification profiles of bacterial genomes.细菌基因组的修饰图谱
Nucleic Acids Res. 1982 Feb 11;10(3):913-34. doi: 10.1093/nar/10.3.913.
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