噬菌体T4基因组的限制酶切图谱。
A restriction map of the bacteriophage T4 genome.
作者信息
O'Farrell P H, Kutter E, Nakanishi M
出版信息
Mol Gen Genet. 1980;179(2):421-435. doi: 10.1007/BF00425473.
Abstract
We report a detailed restriction map of the bacteriophage T4 genome and the alignment of this map with the genetic map. The sites cut by the enzymes Bg/II, XhoI, KpnI, SalI, PstI, EcoRI and HindIII have been localized. Several novel approaches including two-dimensional (double restriction) electrophoretic separations were used.
摘要
我们报告了噬菌体T4基因组的详细限制酶切图谱以及该图谱与遗传图谱的比对情况。已确定了被Bg/II、XhoI、KpnI、SalI、PstI、EcoRI和HindIII等酶切割的位点。使用了包括二维（双重限制酶切）电泳分离在内的几种新方法。
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