Brunel F, Davison J, Merchez M
Gene. 1979 Dec;8(1):53-68. doi: 10.1016/0378-1119(79)90007-6.
Bacteriophage T5 was digested with the restriction endonucleases HindIII and EcoRI and the resulting fragments were inserted into the plasmid pBR322 and the bacteriophage lambda gtWES as vectors. Approx. 15% of the phage genome was recovered in recombinant clones. The recombinants were characterized by restriction analysis, DNA/DNA hybridization employing Southern blots, and ability to complement or recombine with amber mutants of T5. The results obtained allow revisions of the physical map of the T5 genome and partial correlation of the physical map with the genetic map.
用限制性内切酶HindIII和EcoRI消化噬菌体T5，将所得片段插入质粒pBR322和噬菌体λgtWES作为载体。约15%的噬菌体基因组在重组克隆中得到回收。通过限制性分析、采用Southern印迹的DNA/DNA杂交以及与T5琥珀突变体互补或重组的能力对重组体进行表征。所获得的结果允许对T5基因组的物理图谱进行修订，并使物理图谱与遗传图谱部分相关。
