Chu G, Sharp P A
Nature. 1981 Jan 29;289(5796):378-82. doi: 10.1038/289378a0.
A gene chimaera of the first exon from simian virus 40 (SV40) sequences coding for T antigen placed upstream from the third exon from mouse beta-globin, and separated by the resultant new chimaeric intron, was cloned into a bacterial plasmid. When transfected into monkey cells, the gene chimaera was transcribed, polyadenylated and spliced using the donor splice site from SV40 and the acceptor splice site from mouse beta-globin. This result suggests that a donor site from one gene can be spliced to an acceptor site from another gene.
将编码T抗原的猿猴病毒40（SV40）序列的第一个外显子的基因嵌合体置于小鼠β-珠蛋白第三个外显子的上游，并被由此产生的新嵌合内含子隔开，然后克隆到细菌质粒中。当转染到猴细胞中时，该基因嵌合体利用SV40的供体剪接位点和小鼠β-珠蛋白的受体剪接位点进行转录、聚腺苷酸化和剪接。这一结果表明，一个基因的供体位点可以与另一个基因的受体位点进行剪接。
