Southern P J, Howard B H, Berg P
J Mol Appl Genet. 1981;1(3):177-90.
A cDNA segment coding for rabbit beta-globin has been inserted at different locations in the early region of simian virus 40 (SV40). The inserted sequences in these recombinants are transcribed from the SV40 early region promoter, and the primary transcripts are processed to mature mRNAs using viral intervening sequences and the early region polyadenylation site. After infection with various recombinants, beta-globin polypeptide is synthesized only when the beta-globin translation initiation codon is the first AUG in the messenger RNA sequence. When the early region transcripts contain the beta-globin cDNA sequence 3-proximal to the small t antigen coding sequence, beta-globin synthesis is not detectable. However, these recombinants produce small t antigen and abbreviated forms of large T antigen.
编码兔β-珠蛋白的cDNA片段已被插入到猴病毒40（SV40）早期区域的不同位置。这些重组体中的插入序列由SV40早期区域启动子转录，初级转录本利用病毒间隔序列和早期区域聚腺苷酸化位点加工成成熟的mRNA。用各种重组体感染后，只有当β-珠蛋白翻译起始密码子是信使RNA序列中的第一个AUG时，才会合成β-珠蛋白多肽。当早期区域转录本包含小t抗原编码序列3'近端的β-珠蛋白cDNA序列时，无法检测到β-珠蛋白的合成。然而，这些重组体会产生小t抗原和大T抗原的缩短形式。
