用SV40β-珠蛋白重组基因组感染培养的猴肾细胞后兔β-珠蛋白的合成。

Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.

作者信息

Mulligan R C, Howard B H, Berg P

出版信息

Nature. 1979 Jan 11;277(5692):108-14. doi: 10.1038/277108a0.

Abstract

Rabbit beta-globin complementary DNA (cDNA) has been inserted into SV40 DNA in place of the gene coding for the virus' major capsid protein, VP1. The recombinant genome, SVGT5-RabetaG, multiplies efficiently in CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNAs containing the beta-globin coding sequence. Cells propagating SVGT5-RabetaG produce substantial quantities of rabbit beta-globin polypeptide.

摘要

兔β-珠蛋白互补DNA（cDNA）已被插入到SV40 DNA中，取代了编码病毒主要衣壳蛋白VP1的基因。重组基因组SVGT5-RabetaG在CV1猴肾细胞培养物中能高效增殖，并被转录产生含有β-珠蛋白编码序列的细胞质多聚腺苷酸化杂交mRNA。繁殖SVGT5-RabetaG的细胞会产生大量的兔β-珠蛋白多肽。