Bondarenko T P, Belous A M
Ukr Biokhim Zh (1978). 1978 Jan-Feb;50(1):57-9.
The phospholipid composition of mitochondria membranes subjected to rapid freezing (300-400 degrees C per 1 min) to -196 degrees C and subsequent slow warning (at 20 degrees C) was determined by the method of chromatography in the thin layer of silicagel. Under such conditions of freezing and warming a significant decrease in lecithin and ethanolamine phosphatide content is observed in the mitochondria membranes. When freezing the suspension of mitochondria in the medium containing Na2S2O4 in a concentration of 0.05 M only the lipid component changes slightly.
采用硅胶薄层色谱法测定了线粒体膜的磷脂组成，这些线粒体膜先快速冷冻（每分钟300 - 400摄氏度）至 - 196摄氏度，随后缓慢升温（在20摄氏度下）。在这种冷冻和升温条件下，观察到线粒体膜中卵磷脂和乙醇胺磷脂含量显著降低。当在含有0.05 M浓度的Na2S2O4的培养基中冷冻线粒体悬浮液时，只有脂质成分略有变化。
