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[在硫代硫酸钠存在下冻融后线粒体磷脂组成的变化]

[Changes in phospholipid composition of mitochondria after freezing-warming in the presence of Na2S204].

作者信息

Bondarenko T P, Belous A M

出版信息

Ukr Biokhim Zh (1978). 1978 Jan-Feb;50(1):57-9.

PMID:625811
Abstract

The phospholipid composition of mitochondria membranes subjected to rapid freezing (300-400 degrees C per 1 min) to -196 degrees C and subsequent slow warning (at 20 degrees C) was determined by the method of chromatography in the thin layer of silicagel. Under such conditions of freezing and warming a significant decrease in lecithin and ethanolamine phosphatide content is observed in the mitochondria membranes. When freezing the suspension of mitochondria in the medium containing Na2S2O4 in a concentration of 0.05 M only the lipid component changes slightly.

摘要

采用硅胶薄层色谱法测定了线粒体膜的磷脂组成,这些线粒体膜先快速冷冻(每分钟300 - 400摄氏度)至 - 196摄氏度,随后缓慢升温(在20摄氏度下)。在这种冷冻和升温条件下,观察到线粒体膜中卵磷脂和乙醇胺磷脂含量显著降低。当在含有0.05 M浓度的Na2S2O4的培养基中冷冻线粒体悬浮液时,只有脂质成分略有变化。

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