Bondarenko T P, Belous A M
Ukr Biokhim Zh. 1977 Mar-Apr;49(2):30-3.
The phospholipid composition of the membranes of the albino rat liver mitochondria was studied by the method of thin-layer chromatography before and after rapid freezing down to --196 degree C and rapid (at 37 degree C) and slow (at room temperature) thawing. Significant reduction of lecithine and ethanolamine phosphatide composition is observed in the mitochondria exposed to rapid freezing and slow thawing. A simultaneous accumulation of diglycerides is found in the membranes. No reliable changes in phospholipid composition of the mitochondria membranes are observed after exposure to rapid freezing and rapid thawing.
采用薄层色谱法，研究了白化大鼠肝线粒体膜在快速冷冻至-196℃前后以及快速（37℃）和缓慢（室温）解冻后的磷脂组成。在经历快速冷冻和缓慢解冻的线粒体中，观察到卵磷脂和乙醇胺磷脂组成显著减少。同时在膜中发现甘油二酯积累。在经历快速冷冻和快速解冻后，未观察到线粒体膜磷脂组成有可靠变化。
