介导遗传元件重组的哺乳动物细胞功能。
Mammalian cell function mediating recombination of genetic elements.
作者信息
Upcroft P, Carter B, Kidson C
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5835-44. doi: 10.1093/nar/8.23.5835.
Abstract
Recombination of segments of the SV40 genome by a variety of mechanisms is described. These include the faithful joining of linear segments that have flush termini as opposed to previously described cohesive or resected termini. Lack of involvement of viral proteins has been demonstrated for recombination of segments with homologous overlapping termini, but probably applies also to the other joining reactions. Segments of the genome that have been cleaved in such a manner as to be unable to manufacture any known viral proteins are neutral elements of genetic information, incapable of selection by replication or biological function until recombined. These recombination functions presumably are available to the host cell and any element of genetic information that can be generated in that cell.
摘要
描述了通过多种机制对SV40基因组片段进行的重组。这些机制包括线性片段的忠实连接，这些线性片段具有平齐末端，这与先前描述的粘性末端或切除末端相反。对于具有同源重叠末端的片段的重组，已证明病毒蛋白未参与其中，但这可能也适用于其他连接反应。以无法产生任何已知病毒蛋白的方式切割的基因组片段是遗传信息的中性元件，在重组之前无法通过复制或生物学功能进行选择。这些重组功能大概可用于宿主细胞以及该细胞中可产生的任何遗传信息元件。
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本文引用的文献
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Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.利用具有同源重叠末端的SV40基因组片段分析哺乳动物细胞中的重组。
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