Patek E, Johannisson E, Krauer F, Riotton G
Anal Quant Cytol. 1980 Dec;2(4):264-71.
Material obtained from 24 women with benign and malignant breast lesions was subjected to comparative morphologic and cytophotometric DNA analysis. All the cases of benign tumors, as well as benign tissue samples obtained from tumor-containing breasts, exhibited DNA values corresponding to the diploid control value. The tumors classified as carcinoma in situ or highly differentiated invasive cancers (grade I) showed a distribution of the DNA values significantly different from the diploid control values. The tumors classified s grade II and III showed a shift to the "triploid" area or were totally aneuploid. The results of the present pilot study suggest that quantitative cytophotometric DNA analysis can be used for grading the malignancy of breast tumors in combination with conventional histologic examination. Additional studies of larger groups of women may yield further information on the microfluorometric grading of malignancy. Such studies may also contribute to the further development and use of automated methods of cytologic diagnosis.
从24名患有良性和恶性乳腺病变的女性身上获取的材料进行了形态学和细胞光度学DNA分析比较。所有良性肿瘤病例以及从含肿瘤乳房获取的良性组织样本，其DNA值均与二倍体对照值相符。被归类为原位癌或高分化浸润性癌（I级）的肿瘤，其DNA值分布与二倍体对照值有显著差异。被归类为II级和III级的肿瘤显示向“三倍体”区域偏移或完全为非整倍体。本初步研究结果表明，定量细胞光度学DNA分析可与传统组织学检查相结合用于乳腺肿瘤恶性程度分级。对更多女性群体的进一步研究可能会提供有关恶性程度微荧光分级的更多信息。此类研究也可能有助于细胞诊断自动化方法的进一步发展和应用。
