Ogawa N, Yamawaki Y, Kuroda H, Ofuji T, Itoga E, Kito S
Brain Res. 1981 Jan 26;205(1):169-74. doi: 10.1016/0006-8993(81)90728-9.
Thyrotropin releasing hormone (TRH) directly influences central nervous system (CNS) function, independent of its pituitary action. Although these CNS effects have been behaviorally characterized, information is not yet available on the precise regional distribution of its receptor. TRH receptor binding was examined in the monkey CNS by the radioreceptor assay for clarifying the site of TRH action. TRH was bound to brain tissue membranes via high-affinity (Kd = 5.9 x 10(-9) M) and low-affinity (Kd = 11.2 x 10(-8) M) components. TRH receptor binding varied dramatically throughout the monkey brain, with more than 40-fold variation. The limbic system contained the greatest amount of binding. The next highest areas were the cerebral cortex, hypothalamus, interpeduncular nucleus and periaqueductal gray matter of the midbrain. Receptor binding was very low or not detectable in the medial thalamus, cerebellum, brain stem, spinal cord and white matter. These data suggest that TRH has an effect on the CNS via limbic system, cerebral cortex and midbrain.
促甲状腺激素释放激素（TRH）直接影响中枢神经系统（CNS）功能，与其对垂体的作用无关。尽管这些中枢神经系统效应已通过行为学进行了表征，但关于其受体的精确区域分布的信息尚不可得。通过放射受体分析法在猴中枢神经系统中检测TRH受体结合情况，以阐明TRH的作用位点。TRH通过高亲和力（Kd = 5.9×10⁻⁹ M）和低亲和力（Kd = 11.2×10⁻⁸ M）成分与脑组织膜结合。TRH受体结合在整个猴脑中差异极大，变化超过40倍。边缘系统的结合量最大。其次是大脑皮层、下丘脑、脚间核和中脑导水管周围灰质。在内侧丘脑、小脑、脑干、脊髓和白质中，受体结合非常低或无法检测到。这些数据表明，TRH通过边缘系统、大脑皮层和中脑对中枢神经系统产生影响。
