Cheitlin R, Ramachandran J
J Biol Chem. 1981 Apr 10;256(7):3156-8.
Corticotropin (ACTH) induces a characteristic retraction of rat adrenocortical cells in culture. Densitometric analysis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of cell lysates showed that ACTH induced a 20% decrease in actin content. These results were fully confirmed by quantitation of actin by the DNase 1 inhibition assay. The decrease in actin is hormone-specific, concentration-dependent, and correlates temporally with the morphological change induced by ACTH. The change in actin content and the reorganization of microfilament ultrastructure may together mediate hormone regulation of cell shape at the cytoskeletal level.
促肾上腺皮质激素（ACTH）可诱导培养的大鼠肾上腺皮质细胞发生特征性收缩。对细胞裂解物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶进行光密度分析显示，ACTH可使肌动蛋白含量降低20%。通过DNase 1抑制试验对肌动蛋白进行定量分析，充分证实了这些结果。肌动蛋白的减少具有激素特异性、浓度依赖性，并且在时间上与ACTH诱导的形态变化相关。肌动蛋白含量的变化和微丝超微结构的重组可能共同在细胞骨架水平介导激素对细胞形状的调节。
