Hennigar R A, Sens D A, Spicer S S, Schulte B A, Newman V, Sens M A, Garvin A J
Histochem J. 1985 Oct;17(10):1091-110. doi: 10.1007/BF01002535.
Paraffin sections of seven cases of nephroblastoma and one case of clear cell sarcoma were stained with a battery of eleven lectins conjugated to horseradish peroxidase. Lectin staining revealed similarities between blastema and stroma with respect to their content of glycoconjugates whereas blastema and epithelial cells exhibited major differences. In general, blastema and stroma contained glycoconjugates with terminal or penultimate beta-galactose, glycoconjugates having either biantennary or triantennary N-linked sugar chains or both, sialoglycoconjugates, and occasionally glycogen. Epithelial cells also showed these complex carbohydrates but stained additionally for terminal disaccharide galactose-(beta 1----3)-N-acetylgalactosamine, terminal alpha-galactose and terminal alpha-N-acetylgalactosamine. Furthermore, staining with three fucose-binding lectins revealed that the linkage between terminal alpha-fucose residues to the constituent oligosaccharide chains varied between epithelial cells, blastema and stroma. In general, the distribution and content of glycoconjugates in tumour cells comprising clear cell sarcoma resembled that in blastema and stroma of nephroblastoma. Other findings included differences in content of glycosubstance between cuboidal and columnar cells within the same tumour. Also observed were variations between a primary tumour and its metastasis with respect to the occurrence of certain complex carbohydrates.
对7例肾母细胞瘤和1例透明细胞肉瘤的石蜡切片用11种与辣根过氧化物酶偶联的凝集素进行染色。凝集素染色显示，胚基和基质在糖缀合物含量方面具有相似性，而胚基和上皮细胞则表现出主要差异。一般来说，胚基和基质含有带有末端或倒数第二个β-半乳糖的糖缀合物、具有双天线或三天线N-连接糖链或两者都有的糖缀合物、唾液酸糖缀合物，偶尔还有糖原。上皮细胞也显示出这些复杂碳水化合物，但还额外染色显示末端二糖半乳糖-(β1----3)-N-乙酰半乳糖胺、末端α-半乳糖和末端α-N-乙酰半乳糖胺。此外，用三种结合岩藻糖的凝集素染色显示，上皮细胞、胚基和基质中末端α-岩藻糖残基与组成寡糖链之间的连接有所不同。一般来说，透明细胞肉瘤肿瘤细胞中糖缀合物的分布和含量与肾母细胞瘤的胚基和基质中的相似。其他发现包括同一肿瘤内立方细胞和柱状细胞之间糖物质含量的差异。还观察到原发性肿瘤及其转移灶在某些复杂碳水化合物出现方面的差异。
