Bader J P, Brown N R, Ray D A
Cancer Res. 1981 May;41(5):1702-9.
The increased rate of glucose uptake found in cells transformed by Rous sarcoma virus was shown to be enhanced relative to the changes in uptake induced in nontransformed cells by deprivation of glucose (deprivation derepression). Glucose-specific uptake sites were distinguished from glucose-galactose sites in nontransformed cells, and the capacities for glucose uptake and for galactose uptake were increased to about the same extent by the exclusion of glucose from the cell culture medium. Deprivation derepression occurred without a requirement for new RNA or protein synthesis, suggesting that preexisting inactivate uptake sites were activated. Deprivation derepression could be mimicked by the treatment of cells with adenosine triphosphatase activators, and adenosine triphosphate levels were reduced in glucose-deprived cells and in cells treated with adenosine triphosphatase activators. Cells transformed by the Bryan strain of Rous sarcoma virus were unresponsive to addition of high concentrations of glucose, to glucose starvation, or to treatment with adenosine triphosphatase activators, and the relative capacity for glucose uptake in these transformed cells was enhanced much more than the capacity of galactose uptake. It was concluded that cells infected by the Bryan strain of rous sarcoma virus in the process of transformation selectively synthesize more sites specific for glucose uptake. Lower levels of adenosine triphosphate found in transformed cells possibly contribute to a chronic derepression of uptake sites.
劳氏肉瘤病毒转化的细胞中葡萄糖摄取速率的增加，相对于非转化细胞因葡萄糖剥夺（剥夺去抑制）诱导的摄取变化而言有所增强。在非转化细胞中，葡萄糖特异性摄取位点与葡萄糖 - 半乳糖位点得以区分，通过从细胞培养基中排除葡萄糖，葡萄糖摄取能力和半乳糖摄取能力增加到大致相同的程度。剥夺去抑制的发生无需新的RNA或蛋白质合成，这表明预先存在的失活摄取位点被激活。用三磷酸腺苷酶激活剂处理细胞可模拟剥夺去抑制，并且在葡萄糖剥夺的细胞和用三磷酸腺苷酶激活剂处理的细胞中三磷酸腺苷水平降低。由劳氏肉瘤病毒的布莱恩株转化的细胞对添加高浓度葡萄糖、葡萄糖饥饿或用三磷酸腺苷酶激活剂处理无反应，并且这些转化细胞中葡萄糖摄取的相对能力比半乳糖摄取能力增强得更多。得出的结论是，在转化过程中被劳氏肉瘤病毒的布莱恩株感染的细胞选择性地合成了更多对葡萄糖摄取特异的位点。在转化细胞中发现的较低水平的三磷酸腺苷可能导致摄取位点的慢性去抑制。
