Krüse J, Hemminga M A
Eur J Biochem. 1981 Jan;113(3):575-9. doi: 10.1111/j.1432-1033.1981.tb05101.x.
Cowpea chlorotic mottle virus has been labelled with a maleimide spin label when the nucleoprotein particles were in the swollen state. After the spin-labelling reaction, the virus was dialyzed to bring the nucleoprotein particles into different conformational states. The mobility of the labelled part of the protein was monitored by electron paramagnetic resonance (EPR) spectroscopy and by the saturation-transfer EPR technique. The latter technique especially showed sensitivity to conditions that influence the RNA conformation and the RNA-protein interaction. The shape of the EPR and saturation-transfer EPR spectra can only be explained by an anisotropic motion of the whole spin-labelled viral protein or the spin-labelled part of the protein subunit in the core of the virus.
当核蛋白颗粒处于肿胀状态时，豇豆褪绿斑驳病毒已用马来酰亚胺自旋标记物进行了标记。自旋标记反应后，对病毒进行透析，使核蛋白颗粒进入不同的构象状态。通过电子顺磁共振（EPR）光谱和饱和转移EPR技术监测蛋白质标记部分的流动性。后一种技术尤其对影响RNA构象和RNA-蛋白质相互作用的条件表现出敏感性。EPR和饱和转移EPR光谱的形状只能通过整个自旋标记病毒蛋白或病毒核心中蛋白质亚基的自旋标记部分的各向异性运动来解释。
