Danner D B, Deich R A, Sisco K L, Smith H O
Gene. 1980 Nov;11(3-4):311-8. doi: 10.1016/0378-1119(80)90071-2.
Only certain DNA fragments are taken up efficiently by component Haemophilus cells; this implies that efficient uptake requires the presence of a specific nucleotide sequence on the incoming DNA (Sisco and Smith, 1979). To determine the structure of this "uptake site", we have isolated and sequenced four small fragments of cloned H. parainfluenzae DNA which retain the ability to be taken up by cells. These fragments have a sequence of eleven base pairs in common, 5'-AAGTGCGGTCA-3' and ethylation of certain phosphoryl groups in this sequence causes significant decreases in fragment uptake. We conclude that this is the sequence of the uptake site.
只有某些DNA片段能被副流感嗜血杆菌细胞有效摄取；这意味着有效摄取需要进入的DNA上存在特定的核苷酸序列（西斯科和史密斯，1979年）。为了确定这个“摄取位点”的结构，我们分离并测序了克隆的副流感嗜血杆菌DNA的四个小片段，这些片段保留了被细胞摄取的能力。这些片段有一个共同的11个碱基对的序列，5'-AAGTGCGGTCA-3'，并且该序列中某些磷酸基团的乙基化会导致片段摄取显著减少。我们得出结论，这就是摄取位点的序列。
