花椰菜花叶病毒天然DNA和克隆DNA的感染性
Infectivities of native and cloned DNA of cauliflower mosaic virus.
作者信息
Lebeurier G, Hirth L, Hohn T, Hohn B
出版信息
Gene. 1980 Dec;12(1-2):139-46. doi: 10.1016/0378-1119(80)90024-4.
Infectivity assays on turnips reveal that (i) cauliflower mosaic virus (CaMV) DNA, whether circular or linear, is as infectious as the complete virus; (ii) linear DNA obtained with restriction enzymes from the native CaMV DNA has the same specific infectivity as when first cloned in plasmid (pBR322) or bacteriophage (lambda gtWES) vectors and then restricted at the cloning site; (iii) in all cases studied mosaic symptoms are accompanied by virus production. DNA isolated from these viruses is again circular and possesses the three "gaps" characteristic of CaMV DNA. The cloned CaMV DNA, when linked to the vector DNA, is noninfectious or exhibits very low infectivity.
对芜菁进行的感染性检测表明
（i）花椰菜花叶病毒（CaMV）DNA，无论是环状还是线性，其感染性与完整病毒相同；（ii）用限制性内切酶从天然CaMV DNA获得的线性DNA，其比活性与最初克隆到质粒（pBR322）或噬菌体（λgtWES）载体中，然后在克隆位点进行酶切时相同；（iii）在所有研究的案例中，花叶症状都伴随着病毒产生。从这些病毒中分离出的DNA再次呈环状，并具有CaMV DNA特有的三个“缺口”。克隆的CaMV DNA与载体DNA连接后无感染性或感染性极低。
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