Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, rue Descartes, 67084 Strasbourg Cédex, France.
EMBO J. 1984 Jun;3(6):1423-7. doi: 10.1002/j.1460-2075.1984.tb01987.x.
We have used electron microscopy of thin sections and experiments on isolated viroplasms to compare the properties of four strains of cauliflower mosaic virus (CaMV), three of which were partially or completely deleted in open reading frame (ORF) II. Our results confirm that this gene is required for aphid transmissibility and show that the product of ORF II influences the firmness with which virions are held within the viroplasm. Analysis of the proteins in the viroplasms showed that a mutant with a partial deletion in ORF II produced a protein smaller than the normal ORF product. This smaller protein was non-functional with respect both to aphid transmissibility and properties of the viroplasms.
我们使用超薄切片的电子显微镜观察和离体研究包含体的实验，比较了花椰菜花叶病毒（CaMV）的 4 种毒株的性质，其中 3 种毒株在开放阅读框（ORF）II 中部分或完全缺失。我们的结果证实，该基因是蚜虫传播所必需的，并表明 ORF II 的产物影响病毒粒子在包含体内的牢固程度。对包含体中的蛋白质进行分析表明，ORF II 部分缺失的突变体产生的蛋白质小于正常 ORF 产物。这种较小的蛋白质在蚜虫传播性和包含体性质方面均无功能。
