与小鼠免疫球蛋白分泌型μ链对应的克隆cDNA的核苷酸序列。
Nucleotide sequence of a cloned cDNA corresponding to secreted mu chain of mouse immunoglobulin.
作者信息
Auffray C, Rougeon F
出版信息
Gene. 1980 Dec;12(1-2):77-86. doi: 10.1016/0378-1119(80)90017-7.
PMID:6260591
Abstract
The cDNA complementary to a mouse immunoglobulin mu heavy chain mRNA has been cloned into the PstI site of the plasmid vector pBR322. A hybrid plasmid pmu 183.5 containing a 1850 bp insert has been selected by differential screening. The nucleotide sequence of the inset encodes the four constant domains, the terminal piece and the 3'-untranslated region.
摘要
与小鼠免疫球蛋白μ重链mRNA互补的cDNA已被克隆到质粒载体pBR322的PstI位点。通过差异筛选选择了一个含有1850 bp插入片段的杂交质粒pmu 183.5。插入片段的核苷酸序列编码四个恒定结构域、末端片段和3'非翻译区。
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