Schreier P H, Quester S, Bothwell A
Nucleic Acids Res. 1986 Mar 11;14(5):2381-9. doi: 10.1093/nar/14.5.2381.
We report the complete DNA sequence of a c-DNA clone of the heavy chain mu b allele of the C57BL/6 mouse. Comparisons have been made with the nucleotide sequences of the germ line BALB/c mu a and the plasmacytoma TEPC-183 mu a alleles reported elsewhere over the entire length of the coding and the 3' untranslated region. In contrast to the extensive differences between the gamma 2a a and b alleles we have reported earlier we see a very high degree of homology between the mu alleles. Only one of the nucleotide differences between C57BL/6 mu b and BALB/c mu a leads to an amino acid substitution. This single amino acid exchange must form the allotypic determinant of the mu b allele. A comparison of four different DNA sequences indicates that they are all distinct IgM alleles.
我们报告了C57BL/6小鼠重链μb等位基因的一个cDNA克隆的完整DNA序列。已将其与别处报道的种系BALB/c μα和浆细胞瘤TEPC-183 μα等位基因在编码区和3'非翻译区的全长核苷酸序列进行了比较。与我们之前报道的γ2a a和b等位基因之间的广泛差异不同,我们发现μ等位基因之间具有高度的同源性。C57BL/6 μb和BALB/c μα之间的核苷酸差异中只有一个导致了氨基酸替换。这一单氨基酸交换必定构成了μb等位基因的同种异型决定簇。对四个不同DNA序列的比较表明它们都是不同的IgM等位基因。