小鼠颌下腺肾素cDNA片段的分子克隆

Rougeon F, Chambraud B, Foote S, Panthier J J, Nageotte R, Corvol P

Proc Natl Acad Sci U S A. 1981 Oct;78(10):6367-71. doi: 10.1073/pnas.78.10.6367.

The mRNA encoding mouse renin has been partially purified from total poly(A)-containing RNA of submaxillary glands of male Swiss mice. Corresponding cDNAs were cloned in the Pst I site of pBR322. Recombinants have been characterized by differential screening and hybrid-arrested translation. The DNA of clone pRn3-5 has been used to study the expression of renin mRNA in the submaxillary gland and in the kidney of different mouse strains. The renin mRNA from submaxillary gland and kidney have the same length (1600 nucleotides) and appear to be the products of the same gene. In vitro translation of mRNAs and RNA blotting experiments have shown that renin mRNA sequences are accumulated in the submaxillary gland of males of AKR and Swiss strains but not in the gland of male BALB/c.

从小鼠下颌下腺含多聚腺苷酸的总RNA中已部分纯化出编码小鼠肾素的信使核糖核酸（mRNA）。相应的互补脱氧核糖核酸（cDNA）被克隆到pBR322的Pst I位点。重组体已通过差异筛选和杂交阻断翻译进行了鉴定。克隆pRn3 - 5的DNA已用于研究不同小鼠品系下颌下腺和肾脏中肾素mRNA的表达。来自下颌下腺和肾脏的肾素mRNA长度相同（1600个核苷酸），似乎是同一基因的产物。mRNA的体外翻译和RNA印迹实验表明，肾素mRNA序列在AKR和瑞士品系雄性小鼠的下颌下腺中积累，但在雄性BALB/c小鼠的腺体中不积累。