Seta K, Washitake M, Tanaka I, Takai N, Okuyama T
J Chromatogr. 1980 Dec 12;221(2):215-25. doi: 10.1016/s0378-4347(00)84306-6.
A 100-microliter volume of urine was chromatographed on a 50 X 0.4 cm I.D. column packed with a macroreticular anion-exchange resin. Elution was performed with a concave ammonium perchlorate gradient from 0 to 0.25 M at a flow-rate of 0.75 ml/min and a pressure of 7.5-11 MPa. With this perchlorate gradient, no baseline drift occurred in the detection at 254 nm, and even detection at 200 nm was possible. The effect of the addition of ethanol or acetonitrile to the ammonium perchlorate solution was investigated. For the assignment of peaks, ultraviolet spectra of the peaks were measured with stopped-flow scanning spectrophotometry.
取100微升尿液，在一根内径为50×0.4厘米、填充有大孔阴离子交换树脂的柱上进行色谱分析。用从0至0.25M的凹形高氯酸铵梯度以0.75毫升/分钟的流速和7.5 - 11兆帕的压力进行洗脱。使用该高氯酸铵梯度时，在254纳米检测时无基线漂移，甚至在200纳米也能进行检测。研究了向高氯酸铵溶液中添加乙醇或乙腈的影响。为了对峰进行归属，采用停流扫描分光光度法测量了峰的紫外光谱。
