Miyagi H, Miura J, Takata Y, Ganno S
Clin Chem. 1979 Sep;25(9):1617-21.
Several liquid-chromatographic systems involving anion-exchange resins and linear acetate gradient elution have been reported previously for the analysis of ultraviolet-absorbing constituents in human urine. We describe a high-performance liquid-chromatographic system on which a macroreticular anion-exchange resin, and stepwise elution are used. Separations were more rapid with ammonium chloride/acetonitrile stepwise elution than with acetate stepwise elution, and urine constituents could be detected at 225 nm with the former system. Analytical conditions were convenient and the method has proven to be useful for routine clinical analysis.
先前已有多篇报道介绍了几种采用阴离子交换树脂和线性醋酸盐梯度洗脱的液相色谱系统，用于分析人尿中的紫外线吸收成分。我们描述了一种高效液相色谱系统，该系统使用大孔网状阴离子交换树脂和梯度洗脱。氯化铵/乙腈梯度洗脱比醋酸盐梯度洗脱的分离速度更快，并且在前一种系统中可在225nm处检测到尿液成分。分析条件简便，该方法已被证明对常规临床分析有用。
