使用Prepacyte-CB进行脐带血处理比传统的羟乙基淀粉分离法能提高造血祖细胞的可得率。
Umbilical cord blood processing using Prepacyte-CB increases haematopoietic progenitor cell availability over conventional Hetastarch separation.
作者信息
Basford C, Forraz N, Habibollah S, Hanger K, McGuckin C P
机构信息
Newcastle Centre for Cord Blood, Institute of Human Genetics, Newcastle University, Newcastle upon Tyne, UK.
出版信息
Cell Prolif. 2009 Dec;42(6):751-61. doi: 10.1111/j.1365-2184.2009.00646.x. Epub 2009 Sep 15.
BACKGROUND
Currently the most frequently used method for umbilical cord blood separation in many hospitals across the UK and the rest of the world, where small-to-medium amounts of samples are processed, is Hetastarch, a mechanical, starch-based method, which causes red cell agglutination by rouleaux formation.
AIM
In this study, a novel method (Prepa-Cyte-CB), in comparison with Hetastarch as part of an FDA-approved clinical study, was evaluated.
MATERIALS AND METHODS
Validation of data included recovery of nucleated and CD34+ cells, red blood cell reduction, colony forming unit potential, flow cytometric analysis and sterility tests.
RESULTS
PrepaCyte-CB, in comparison with Hetastarch offers fast, reliable separation with improved recovery of nucleated cells, 72.03% (+/-8.48 SD) compared to 58.09% (+/-20.06 SD), and CD34+ haematopoietic progenitor cells, 76% (+/-19.54 SD) compared to 64.19% (+/-29.77 SD). PrepaCyte-CB was also 12-fold more efficient in removing red blood cells and haemoglobin (P < 0.001) than Hetastarch.
CONCLUSIONS
These results show that PrepaCyte-CB offers superior separation of UCB when compared to Hetastarch.
背景
目前，在英国及世界其他地区的许多医院中，当处理中小量样本时，最常用的脐血分离方法是羟乙基淀粉法，这是一种基于淀粉的机械方法，通过红细胞缗钱状形成导致红细胞凝集。
目的
在本研究中，作为美国食品药品监督管理局（FDA）批准的一项临床研究的一部分，对一种新方法（Prepa-Cyte-CB）与羟乙基淀粉法进行了比较评估。
材料与方法
数据验证包括有核细胞和CD34+细胞的回收率、红细胞减少情况、集落形成单位潜力、流式细胞术分析和无菌测试。
结果
与羟乙基淀粉法相比，PrepaCyte-CB能实现快速、可靠的分离，有核细胞回收率更高，分别为72.03%（±8.48标准差）和58.09%（±20.06标准差），CD34+造血祖细胞回收率分别为76%（±19.54标准差）和64.19%（±29.77标准差）。PrepaCyte-CB去除红细胞和血红蛋白的效率也比羟乙基淀粉法高12倍（P < 0.001）。
结论
这些结果表明，与羟乙基淀粉法相比，PrepaCyte-CB在脐血分离方面表现更优。
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