使用不连续的 Percoll 梯度从人骨髓和外周血中分离细胞群体。
The use of discontinuous Percoll gradients to separate populations of cells from human bone marrow and peripheral blood.
作者信息
Ellis W M, Georgiou G M, Roberton D M, Johnson G R
出版信息
J Immunol Methods. 1984 Jan 20;66(1):9-16. doi: 10.1016/0022-1759(84)90242-4.
Cells from human bone marrow samples were separated on discontinuous Percoll gradients and the effects of variations in osmolality were studied. Yield of committed progenitor cells as determined by CFU-c and BFU-E assays was improved when a gradient of discontinuous osmolality (360-320 mosM) was compared with gradients of uniform osmolality (320 mosM and 290 mosM). The CFU-c and BFU-E containing fraction recovered from this gradient also contained fewer T cells. When used for separation of peripheral blood samples, the same gradient yielded populations of mononuclear cells, neutrophils and platelets of more than 90% purity.
将人骨髓样本中的细胞在不连续的 Percoll 梯度上进行分离，并研究渗透压变化的影响。当将不连续渗透压梯度（360 - 320 mosM）与均匀渗透压梯度（320 mosM 和 290 mosM）进行比较时，通过 CFU-c 和 BFU-E 测定法确定的定向祖细胞产量有所提高。从该梯度中回收的含有 CFU-c 和 BFU-E 的部分中 T 细胞也较少。当用于分离外周血样本时，相同的梯度产生纯度超过 90% 的单核细胞、中性粒细胞和血小板群体。
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