Yoshikawa H, Friedmann T, Ito J
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1336-40. doi: 10.1073/pnas.78.3.1336.
The nucleotide sequences of the first 422 base pairs from the left-hand end and the first 274 base pairs from the right-hand end of phi 29 DNA were determined by using the chemical degradation method of Maxam and Gilbert. The data indicate that phi 29 DNA has inverted terminal repetitions that are six base pairs long 5' (-A-A-A-G-T-A-). No perfectly self-complementary sequence exists within the terminal regions of phi 29 DNA, suggesting that DNA replication via a self-priming mechanism is improbable. The putative early promoter sequences were found in both ends of the phi 29 DNA. The results of the sequence determination are discussed in relation ship to models proposed for the mechanism of replication of linear DNA molecules.
采用马克萨姆和吉尔伯特的化学降解法测定了φ29 DNA左手端前422个碱基对以及右手端前274个碱基对的核苷酸序列。数据表明，φ29 DNA具有6个碱基对长（5' -A-A-A-G-T-A-）的反向末端重复序列。在φ29 DNA的末端区域内不存在完全自我互补的序列，这表明通过自我引发机制进行DNA复制不太可能。在φ29 DNA的两端都发现了推定的早期启动子序列。结合为线性DNA分子复制机制提出的模型对序列测定结果进行了讨论。
