腺病毒5型DNA末端的核苷酸序列。
The nucleotide sequence at the termini of adenovirus type 5 DNA.
作者信息
Steenbergh P H, Maat J, van Ormondt H, Sussenbach J S
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4371-89. doi: 10.1093/nar/4.12.4371.
The sequences of the first 194 base pairs at both termini of adenovirus type 5 (Ad5) DNA have been determined, using the chemical degradation technique developed by Maxam and Gilbert (Proc. Nat. Acad. Sci. USA 74 (1977), pp. 560-564). The nucleotide sequences 1-75 were confirmed by analysis of labeled RNA transcribed from the terminal HhaI fragments in vitro. The sequence data show that Ad5 DNA has a perfect inverted terminal repetition of 103 base pairs long.
利用马克萨姆和吉尔伯特开发的化学降解技术（《美国国家科学院院刊》74 (1977)，第560 - 564页），测定了5型腺病毒（Ad5）DNA两端前194个碱基对的序列。通过对体外从末端HhaI片段转录的标记RNA进行分析，证实了核苷酸序列1 - 75。序列数据表明，Ad5 DNA具有一个长度为103个碱基对的完美反向末端重复序列。
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