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人纤维蛋白原Aα链和γ链cDNA克隆的分离与鉴定

Isolation and characterisation of cDNA clones for the A alpha- and gamma-chains of human fibrinogen.

作者信息

Imam A M, Eaton M A, Williamson R, Humphries S

出版信息

Nucleic Acids Res. 1983 Nov 11;11(21):7427-34. doi: 10.1093/nar/11.21.7427.

DOI:10.1093/nar/11.21.7427
PMID:6689067
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326493/
Abstract

cDNA clones coding for the A alpha- and gamma-chains of human fibrinogen have been isolated from an adult liver cDNA library. Clones were identified by hybridisation with mixtures of synthetic oligonucleotides 17 bases long, predicted using amino acid sequence data for each chain. The cDNA insert sizes are 1,950bp for A alpha-fibrinogen and 950bp for gamma-fibrinogen. The clones do not show any cross-hybridisation. Each cDNA hybridises to a unique sequence in the human genome. In adult human liver, Northern blots give an estimated messenger RNA size of 2.6kb for A alpha-fibrinogen and 1.8kb for gamma-fibrinogen.

摘要

已从成人肝脏cDNA文库中分离出编码人纤维蛋白原Aα链和γ链的cDNA克隆。通过与根据每条链的氨基酸序列数据预测的17个碱基长的合成寡核苷酸混合物杂交来鉴定克隆。Aα纤维蛋白原的cDNA插入片段大小为1950bp，γ纤维蛋白原的为950bp。这些克隆未显示任何交叉杂交。每个cDNA与人类基因组中的独特序列杂交。在成人人类肝脏中，Northern印迹显示Aα纤维蛋白原的信使RNA大小估计为2.6kb，γ纤维蛋白原的为1.8kb。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/a593efd5bc7b/nar00366-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/bd66845e3064/nar00366-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/9f9b97bb85ae/nar00366-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/a593efd5bc7b/nar00366-0179-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/bd66845e3064/nar00366-0176-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/9f9b97bb85ae/nar00366-0178-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a2c/326493/a593efd5bc7b/nar00366-0179-a.jpg

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本文引用的文献

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Organization of the rat gamma-fibrinogen gene: alternative mRNA splice patterns produce the gamma A and gamma B (gamma ') chains of fibrinogen.
位于4号染色体上的三个限制性片段长度多态性相对于已知遗传标记的位置。
Hum Genet. 1984;68(2):154-8. doi: 10.1007/BF00279306.
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Am J Hum Genet. 1986 Dec;39(6):787-96.
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