用于从污泥中回收肠道病毒的尿素-赖氨酸法。
Urea-lysine method for recovery of enteroviruses from sludge.
作者信息
Farrah S R, Scheuerman P R, Bitton G
出版信息
Appl Environ Microbiol. 1981 Feb;41(2):455-8. doi: 10.1128/aem.41.2.455-458.1981.
Abstract
Enteroviruses added to sludge and indigenous viruses present in sludge were recovered by treating the sludge flocs with a 4 M urea solution buffered at pH 9 with 0.5 M lysine. Eluted viruses were absorbed to aluminum hydroxide flocs and collected by centrifugation. The flocs were solubilized with 0.1 M ethylenediaminetetraacetic acid-3% beef extract at pH 9. After dialysis to remove the ethylenediaminetraacetic acid, viruses were further concentrated by organic flocculation. Approximately 40% of poliovirus and coxsackievirus B-3 added to 500 to 1,000 ml of sludge could be recovered in final sample volumes of less than 10 ml. Polioviruses, echoviruses, and coxsackieviruses were recovered from different samples of wastewater sludge.
摘要
通过用0.5M赖氨酸在pH9缓冲的4M尿素溶液处理污泥絮凝物，回收添加到污泥中的肠道病毒和污泥中存在的本地病毒。洗脱的病毒被吸附到氢氧化铝絮凝物上并通过离心收集。絮凝物在pH9下用0.1M乙二胺四乙酸-3%牛肉提取物溶解。透析去除乙二胺四乙酸后，病毒通过有机絮凝进一步浓缩。添加到500至1000ml污泥中的脊髓灰质炎病毒和柯萨奇病毒B-3中，约40%可在最终样品体积小于10ml的情况下回收。从不同的废水污泥样品中回收了脊髓灰质炎病毒、埃可病毒和柯萨奇病毒。
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