Brashear D A, Ward R L
Appl Environ Microbiol. 1982 Jun;43(6):1413-8. doi: 10.1128/aem.43.6.1413-1418.1982.
Five general methods for recovering indigenous viruses from raw wastewater sludge were compared. Each method included elution, concentration, and disinfection steps. The elution method, found to consistently yield the greatest viral recovery, was a two-phase technique that involved blending sludge with Freon. Other methods, including two being tested as American Society for Testing Materials tentative standard methods, were less effective. Viral recoveries were generally greater (sometimes much greater) if samples were concentrated by high-speed centrifugation rather than by organic flocculation with 3% beef extract. Three cell lines were used to measure viral recoveries by the plaque assay. The efficiency of recovery was greatest on BGM cells, followed by RD and MA-104 cells.
比较了从原废水污泥中回收本地病毒的五种常规方法。每种方法都包括洗脱、浓缩和消毒步骤。洗脱方法被发现始终能产生最大的病毒回收率，这是一种两相技术，涉及将污泥与氟利昂混合。其他方法，包括两种作为美国材料试验协会暂行标准方法正在测试的方法，效果较差。如果样品通过高速离心而不是用3%牛肉提取物进行有机絮凝浓缩，病毒回收率通常更高（有时高得多）。使用三种细胞系通过噬斑测定法测量病毒回收率。在BGM细胞上回收率最高，其次是RD和MA-104细胞。
