Kostenbader K D, Cliver D O
Appl Microbiol. 1972 Oct;24(4):540-3. doi: 10.1128/am.24.4.540-543.1972.
A simple and rapid method for recovering enteroviruses from oysters is described. A polycation sewage flocculant promoted cohesion of oyster solids and thereby aided separation of these from the viruses. Recovery of 80 to 100% of experimentally inoculated virus was achieved, and the suspension or extract obtained could be inoculated directly into tissue cultures or concentrated first for greater sensitivity.
本文描述了一种从牡蛎中回收肠道病毒的简单快速方法。一种聚阳离子污水絮凝剂促进了牡蛎固体的凝聚，从而有助于将其与病毒分离。实验接种病毒的回收率达到80%至100%，所获得的悬浮液或提取物可直接接种到组织培养物中，或先进行浓缩以提高灵敏度。
