来自地衣芽孢杆菌A.T.C.C. 9945细胞壁的磷壁酸。
The teichuronic acid from the walls of Bacillus licheniformis A.T.C.C. 9945.
作者信息
Lifely M R, Tarelli E, Baddiley J
出版信息
Biochem J. 1980 Nov 1;191(2):305-18. doi: 10.1042/bj1910305.
The teichuronic acid of Bacillus licheniformis A.T.C.C. 9945 grown under phosphate limitation was isolated from the cell walls and purified by ion-exchange and Sephadex chromatography. The detailed structure of the polysaccharide was established by methylation analysis, periodate oxidation and partial acid hydrolysis. The polymer is composed of tetrasaccharide repeating units with the structure [GlcA beta(1 leads to 4)GlcA beta(1 leads to 3)GalNAc beta(1 leads to 6)GalNAc alpha(1 leads to 4)n. 13C n.m.r. analysis has confirmed most of the structural features of the polysaccharide and, in particular, the anomeric configurations and linkage positions of substituents. The teichuronic acid from glucose-limited cells was identical with that from cells grown under phosphate limitation.
在磷酸盐限制条件下生长的地衣芽孢杆菌A.T.C.C. 9945的磷壁醛酸从细胞壁中分离出来，并通过离子交换和葡聚糖凝胶色谱法进行纯化。通过甲基化分析、高碘酸盐氧化和部分酸水解确定了该多糖的详细结构。该聚合物由具有[GlcAβ(1→4)GlcAβ(1→3)GalNAcβ(1→6)GalNAcα(1→4)]n结构的四糖重复单元组成。13C核磁共振分析证实了该多糖的大部分结构特征，特别是取代基的端基构型和连接位置。来自葡萄糖限制细胞的磷壁醛酸与在磷酸盐限制条件下生长的细胞中的磷壁醛酸相同。
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