肺炎链球菌细胞壁中C-多糖的结构。
The structure of C-polysaccharide from the walls of Streptococcus pneumoniae.
作者信息
Poxton I R, Tarelli E, Baddiley J
出版信息
Biochem J. 1978 Dec 1;175(3):1033-42. doi: 10.1042/bj1751033.
Abstract
The well-known immologically active component of pneumococci, C-polysaccharide, is a teichoic acid that can be isolated from the cell walls and purified by Sephadex and ion-exchange chromatography. Further details of the structure of C-teichoic acid were established by chemical degradation, including hydrolysis in acid and alkali, treatment with HF, periodate oxidation and methylation. In addition, the use of 13C n.m.r. has confirmed some of these structural features and resulted in a proposal for the order of substituents, the location of positions of substitution and the configuration of anomeric centres in the repeating unit of the polymer.
摘要
肺炎球菌中著名的免疫活性成分C多糖是一种磷壁酸，可从细胞壁中分离出来，并通过葡聚糖凝胶和离子交换色谱法进行纯化。通过化学降解，包括在酸和碱中水解、用氢氟酸处理、高碘酸盐氧化和甲基化，确定了C磷壁酸结构的更多细节。此外，13C核磁共振的使用证实了其中一些结构特征，并得出了聚合物重复单元中取代基顺序、取代位置和异头中心构型的提议。
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