Solnick D, Anderson M A
J Virol. 1982 Apr;42(1):106-13. doi: 10.1128/JVI.42.1.106-113.1982.
A adenovirus type 5 host range mutant (hr440) has been isolated which is defective in a splicing event required to generate the middle-sized mRNA from early region 1A. This defect has been ascribed to two adjacent nucleotide changes which lie five and six nucleotides from the 5' splice site for this mRNA (Solnick, Nature 291:508-510, 1981). One of these changes introduces an amber codon into the reading frame of the largest region 1A mRNA, resulting in the production of a truncated polypeptide. Like other region 1A mutants, hr440 is defective in the production of mRNA from early regions 2 and 3, but hr440 is unusual in that transcription from regions 1B and 4 is normal. Furthermore, although region 1B expression is unaffected, hr440 does not transform baby rat kidney cells. Therefore, expression of early region 1B is insufficient for transformation, eliminating the possibility that region 1A is required only to induce such expression.
已分离出一种5型腺病毒宿主范围突变体（hr440），它在从早期区域1A产生中等大小mRNA所需的剪接事件中存在缺陷。这种缺陷归因于两个相邻的核苷酸变化，它们位于该mRNA的5'剪接位点的五个和六个核苷酸处（索尔尼克，《自然》291:508 - 510，1981）。其中一个变化在最大的区域1A mRNA的阅读框中引入了一个琥珀密码子，导致产生截短的多肽。与其他区域1A突变体一样，hr440在从早期区域2和3产生mRNA方面存在缺陷，但hr440的不同寻常之处在于，来自区域1B和4的转录是正常的。此外，尽管区域1B的表达不受影响，但hr440不能转化幼鼠肾细胞。因此，早期区域1B的表达不足以实现转化，排除了仅需要区域1A来诱导这种表达的可能性。
